RESUMO
Objective To explore the correlation of circRNAs' expression level to the negative-and positive symptoms of patients with schizophrenia (SZ).Methods Gene chip screening was performed with the peripheral blood samples from each five of SZ patients and normal controls.Nine circRNAs showing differentiate expression were confirmed,and further verification was done by real-time fluorescence quantitative PCR in 102 SZ patients and 103 normal controls.All the SZ patients were assessed with Positive and Negative Symptom Scale (PANSS).Results It was revealed that the expression levels of circRNA_102101,circRNA_102315,circRNA_104597,circRNA_101835 and circRNA_101836 were significantly down-regulated (P＜0.01 or P＜0.05),and circRNA_103102 and circRNA_103704 were up-regulated in SZ group (P＜0.01).The ACT value of circRNA_102101 and circRNA_103102 was positively correlated to the positive symptoms (P＜0.01 or P＜0.05),and the ACT value of circRNA_103704 also showed positive correlation with positive symptoms and general psychopathological symptoms (P＜0.01 or P＜0.05).The ACT values of circRNA_102101,circRNA_103102,circRNA_102315,circRNA_103704 and circRNA_102802 were correlated with thinking disorder (P＜0.01 or P＜0.05),and the ACT values of circRNA_102101,circRNA_103102,circRNA_104597,circRNA_103704 and circRNA_102802 were correlated with the activation (P＜0.01 or P＜0.05).The ACT values of circRNA_102101,circRNA_103102,circRNA_103704 and circRNA_102802 were positively correlated with paranoid (P＜0.01 or P＜0.05),and of circRNA_102101,circRNA_103102,circRNA_103704 and circRNA_102802 were markedly correlated with assault (P＜0.01 or P＜0.05).Therefore,circRNA_103704 was chosen into regressive equation of positive symptoms (P＜0.01),and circRNA_103704 and circRNA_102315 were chosen into regressive equation of general pathological findings (P＜0.01 or P＜0.05).Conclusion The expression levels of circRNA_103704 and circRNA_103102 are obviously up-regulated in SZ patients than in normal controls,and markedly correlated with the negative and positive SZ symptoms,so might be the dominant regulatory factors in the pathological process of schizophrenia.